J. Nucl. Phy. Mat. Sci. Rad. A.

Effects of Low-Energy Laser Irradiation on Sperm Cells Dynamics of Rabbit (Oryctolagus Cuniculus)

J.M. De Jesús-Miranda, I. A. Mandujano, F. Méndez3, Y.J. Castillo, J. Mulia, C. García, Y.E. Felipe and D. Osorio-González

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sperm motility, photo-biostimulation.

PUBLISHED DATE August 07, 2017
PUBLISHER The Author(s) 2017. This article is published with open access at www.chitkara.edu. in/publications

Infertility is a world disease in which a couple is unable to achieve pregnancy. There are numerous parameters to determinate fertility; nevertheless, sperm motility is by consensus one of the most important attributes to evaluate male fertility. Contributions to a better understanding of this crucial parameter are imperative; hence, the aim of this investigation was to assess the effect of low-energy laser irradiation on sperm cell dynamics in thawed samples that were cryopreserved. We used a 405 nm blue laser beam to irradiate spermatic cells from rabbit inside a temperature-controlled dispersion chamber at 37 °C; then, we applied an image recognizing system to calculate individual sperm trajectories and velocities. We found that sperms raise its motility after irradiation suggesting that λ=405 nm is an optimal wavelength for spermatic photo-stimulation.

Page(s) 187–196
URL http://dspace.chitkara.edu.in/jspui/bitstream/1/878/3/51018_JNP_Osorio-Gonz%c3%a1lez.pdf
ISSN 2321-8649
DOI https://doi.org/10.15415/jnp.2017.51018
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