Using Green Fluorescent Protein to Correlate Temperature and Fluorescence Intensity into Bacterial Systems

K.  Beltran; J. M.  De Jesus-Miranda; J. A.  Castro; L. A.  Mandujano-Rosas; J. M.  Paulin-Fuentes; D.  Osorio-Gonzalez

doi:10.15415/jnp.2016.41005

Authors

K. Beltran Laboratorio de Biofísica Molecular. Facultad de Ciencias de la Universidad Autónoma del Estado de México. Instituto Literario 100, Col. Centro Toluca, Estado de México C.P. 50000. México.
J. M. De Jesus-Miranda Laboratorio de Biofísica Molecular. Facultad de Ciencias de la Universidad Autónoma del Estado de México. Instituto Literario 100, Col. Centro Toluca, Estado de México C.P. 50000. México.
J. A. Castro Laboratorio de Biofísica Molecular. Facultad de Ciencias de la Universidad Autónoma del Estado de México. Instituto Literario 100, Col. Centro Toluca, Estado de México C.P. 50000. México.
L. A. Mandujano-Rosas Laboratorio de Biofísica Molecular. Facultad de Ciencias de la Universidad Autónoma del Estado de México. Instituto Literario 100, Col. Centro Toluca, Estado de México C.P. 50000. México.
J. M. Paulin-Fuentes Laboratorio de Biofísica Molecular. Facultad de Ciencias de la Universidad Autónoma del Estado de México. Instituto Literario 100, Col. Centro Toluca, Estado de México C.P. 50000. México.
D. Osorio-Gonzalez Laboratorio de Biofísica Molecular. Facultad de Ciencias de la Universidad Autónoma del Estado de México. Instituto Literario 100, Col. Centro Toluca, Estado de México C.P. 50000. México.

DOI:

https://doi.org/10.15415/jnp.2016.41005

Keywords:

green fluorescent protein, bacterial systems, bacterial temperature, spectroscopic properties

Abstract

The unique and stunning spectroscopic properties of Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria, not to mention of its remarkable structural stability, have made it one of the most widely studied and used molecular tool in medicine, biochemistry, and cell biology. Its high fluorescent quantum yield is due to its chromophore, structure responsible of emitting green visible light when excited at 395 nm. Although it is noteworthy that there is enormous available information of the wonderful luminescent properties of GFP, the fact is that there are features and properties unexplored yet, particulary about its capabilities as molecular reporter in several biological processes. In this work, we used recombinant DNA technology to express the protein in bacteria; prepared the bacterial system both in liquid and solid media, and assembled an experimental set to expose those media to a laser beam; thereby we excited the protein chromophore and used emission spectroscopy in order to observe variations in fluorescence when the bacterial system is exposed to different temperatures.

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