Potential of Some Fungal and Bacterial Species in Bioremediation of Heavy Metals

Raman  Kumar; Prem  Singh; Bhupinder  Dhir; Anil K.  Sharma; Devinder  Mehta

doi:10.15415/jnp.2014.12017

Authors

Raman Kumar Department of Biotechnology, M.M. University, Mullana-133203, Haryana, India
Prem Singh Department of Physics, S.D. College, Ambala Cantt.-133001, Haryana, India
Bhupinder Dhir Department of Genetics, South Campus, Delhi University, New Delhi-110021, India
Anil K. Sharma Department of Biotechnology, M.M. University, Mullana-133203, Haryana, India
Devinder Mehta Department of Physics, Panjab University, Chandigarh-160014, India

DOI:

https://doi.org/10.15415/jnp.2014.12017

Keywords:

Heavy metals, Biosorption, Wastewater, Fungi, Bacteria

Abstract

Microorganisms including fungi and bacteria have been reported to extract heavy metals from wastewater through bioaccumulation and biosorption. An attempt was, therefore, made to isolate bacteria and fungi from sites contaminated with heavy metals for higher tolerance and removal from wastewater. Bacterial and fungal isolates were obtained from the samples collected from Karnal, Ambala and Yamunanagar districts of Haryana using enrichment culture technique. Bacterial and fungal isolates with tolerant up to 100 ppm concentration of heavy metals (Pb, Cd, Cr) were tested for their removal from liquid media containing 50 ppm concentration of Pb, Cd and Cr each. Five fungi (Penicillium chrysogenum, Aspegillus nidulans, Aspergillus flavus, Rhizopus arrhizus, Trichoderma viride) were also included in this study. Fungi Aspergillus nidulans, Rhizopus arrhizus and Trichoderma viride showed maximum uptake capacity of 25.67 mg/g for Pb, 13.15 mg/g for Cd and 2.55 mg/g of Cr, respectively. The maximum uptake capacity of tolerant bacterial isolates - BPb12 and BPb16, BCd5 and BCr14 were observed to be ~ 45 mg/g for Pb, 2.12 mg/g for Cd and 3.29 mg/g for Cr, respectively. This indicated the potential of these identified fungi and bacteria as biosorbent for removal of high concentration metals from wastewater and industrial effluents.

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References

A. Malik, “Metal bioremediation through growing cells,” Environmental International Journal 30, 261-278 (2004). http://dx.doi.org/10.1016/j.envint.2003.08.001

T.G. Chuah, A. Jumasiah, I. Azni, S. Katayon and S.Y.T. Choong, “Rice husk as a potentially low-cost biosorbent for heavy metal and dye removal: an overview,” Desalination 175, 305-316 (2005). http://dx.doi.org/10.1016/j.desal.2004.10.014

J.A. Brierley, C.L. Brierley, and G.M. Goyak, “AMT-BIOCLAIM: A new wastewater treatment and metal recovery technology. In: Fundamental and Applied Biohydrometallurgy, Eds. R.W. Lawrence, R.M.R. Branion and G. Ebner, Elsevier, Amsterdam 291-303 (1986).

M. Nourbakhsh, Y. Sag, D. Ozer, Z. Aksu, T. Katsal and A. Calgar, “A comparative study of various biosorbents for removal of chromium (VI) ions from industrial wastewater,” Process Biochemistry 29, 1-5 (1994). http://dx.doi.org/10.1016/0032-9592(94)80052-9

L.E. Macaskie, “The application of biotechnology to the treatment of wastes produced from the nuclear fuel cycle: biodegradation and bioaccumulation as a means of treating radionuclide containing streams,” Critical Reviews in Biotechnology 11, 41-112 (1991). http://dx.doi.org/10.3109/07388559109069183

S.A. Churchill, J.V. Walters and P.F. Churchill, “Sorption of heavy metals by prepared bacterial cell surfaces,” Journal of Environmental Engineering 121, 706-711 (1995). http://dx.doi.org/10.1061/(ASCE)0733-9372(1995)121:10(706)

G.M. Gadd, “Fungi and yeasts for metal accumulation,” In Microbial Mineral Recovery, Eds. H.L. Ehrlich and C.L. Brierle, McGraw-Hill, New York, 249-276 (1990).

F. Veglio and F. Beolchini, “Removal of metals by biosorption: a review,” Journal of Hydrometallurgy 74, 301-316 (1997). http://dx.doi.org/10.1016/S0304-386X(96)00059-X

K.M. Elizabeth and T.V.R. Anuradha, “Biosorption of hexavalent chromium by nonpathogenic bacterial cell preparations,” Indian Journal of Microbiology 40, 263-265 (2000).

S.R. Bai, and T.E. Abraham, “Studies on chromium (VI) Adsorption – desorption using immobilized fungal biomass,” Bioresource Technology 87, 17-26 (2003). http://dx.doi.org/10.1016/S0960-8524(02)00222-5

A.E. Greenberg, R.R. Trussell, and L.S. Clesceri, “Standard methods for the examination of water and wastewater, 16th edition,” American Public Health Association, Washington, DC, 146-150 (1985).

S. Solarsk, T. May, F.A. Roddick and A.C. Lawrie, “Isolation and screening of natural organic matter-degrading fungi, Chemosphere 75, 751-8 (2009). http://dx.doi.org/10.1016/j.chemosphere.2009.01.030

V.S.K.V. Rama Rao, N. Akhtar and M.P. Maruthi, “Isolation of a cadmium tolerant Curvularia sp. for polluted effluent,” Current Science 73, 453 (1997).

J.S. Chang, R. Law and C.C. Chang, “Biosorption of lead, copper and cadmium by biomass of Pseudomonas Aeruginosa PU 21,” Water Research 31, 1651–1658 (1997). http://dx.doi.org/10.1016/S0043-1354(97)00008-0

A. Kapoor, T. Viraraghavan and D.R. Cullimore, “Removal of heavy metals using the fungus Aspergillus niger,” Bioresource Technology 70, 95–104 (1999). http://dx.doi.org/10.1016/S0960-8524(98)00192-8

P.R. Puranik and K.M. Paknikar, “Biosorption of lead, cadmium and zinc by Citrobacter strain MCM B-181: characterization studies,” Biotechnology Program 15, 228-37 (1999). http://dx.doi.org/10.1021/bp990002r

A.D. Costa, A. Carlos and F. Pereira, “Bioaccumulation of copper, zinc, cadmium and lead by Bacillus sp., Bacillus cereus, Bacillus sphaericus and Bacillus subtilis,” Brazilian Journal of Microbiology 32, 1-5 (2001).

R. Pardo, M. Herguedas, E. Barrado and M. Vega, “Biosorption of cadmium, copper, lead and zinc by inactive biomass of Pseudomonas putida,” Analytical and Bioanalytical Chemistry 376, 26-32 (2003).

A. Selatnia, A. Boukazoula, B.N. Kechid, M.Z. Bakhti, A. Chergui and Y. Kerchich, “Biosorption of lead (II) from aqueous solution by a bacterial dead Streptomyces rimosus biomass,” Biochemical Engineering Journal 19, 127-135 (2004).

S.B. Choi, and Y.S. Yun, Lead biosorption by waste biomass of Corynebacterium glutamicum generated from lysine fermentation process. Biotechnology Letters 26, 331-336 (2004).

S. Tunali, T. Akar, A. Safa Ozcan, I. Kiran and A. Ozcan,“ Equilibrium and kinetics of biosorption of lead (II) from aqueous solutions by Cephalosporium aphidicola” Separation and Purification Technology 47, 105-112 (2006).

M.I. Kefala, A.I. Zouboulis and K.A. Matis, Biosorption of cadmium ions by actinomycetes and separation by flotation. Environmental Pollution 104, 283-293 (1999).

H.A. Ghoslan, S.A. Sabry, and R.A. Amer, “Bioaccumulation of nickel, cobalt and cadmium by free and immobilized cells of Pseudomonas sp,” Fresenius Environmental Bulletin 8, 428-435 (1999).

R. Say, A. Denizli and M.Y. Arica, “Biosorption of cadmium (II), lead (II) and copper (II) with the filamentous fungus Phanerochaete chrysosporium,” Bioresource Technology 76, 67-70 (2001).

M. Watanabe, K. Kawahara, K. Sasaki and N. Noparatnaraporn, “Biosorption of cadmium ions using a photosynthetic bacterium, Rhodobacter sphaeroides S and a marine photosynthetic bacterium, Rhodovulum sp. and their biosorption kinetics,” Journal of Bioscience and Bioengineering 95, 374-378 (2003).

G. Ozdemir, N. Ceyhan, T. Ozturk, F. Akirmak and T. Cosar, “Biosorption of chromium (VI), cadmium (II) and copper (II) by Panteo sp. TEM18,” Journal of Chemical Engineering 102, 249-253 (2004).

H.Y. Qi, Q. Hu, M.N. Dou and J.H. Zeng, “Biosorption of Cd2+ by a hyper resistant newly isolated Bacillus cereus strain,” In 11th International Symposium on Microbial Ecology Vienna, Austria, Abstract, p 79, (2006).

Z. Aksu, T. Kutsal, S. Gun, N. Haciosmanoglu and M. Gholaminejad, “Investigation of biosorption of Cu (II), Ni (II) and Cr (VI) ions to activated sludge bacteria,” Environmental Technology 12, 915-921 (1991).

C. Solisio, A. Lodi, A. Converti and M. Del Borghi, “The effect of acid pre-treatment on the biosorption of chromium (III) by a Sphaerotilus natans from industrial wastewaters,” Water Research 34, 3171-3178 (2000).

T. Srinath, T. Verma, P.W. Ramteke and S.K. Garg, “Chromium (VI) biosorption and bioaccumulation by chromate resistant bacteria” Chemosphere 48, 427-435 (2002).

A. Iyer, K. Mody and B. Jha, “Accumulation of hexavalent chromium by an exopolysaccharide producing marine Enterobacter cloaceae,” Marine Pollution Bulletin 49, 974-977 (2004). http://dx.doi.org/10.1016/j.marpolbul.2004.06.023

T. Akar, and S. Tunali, “Biosorption characteristics of Aspergillus flavus biomass for removal of Pb (II) and Cu (II) ions from an aqueous solution,” Bioresource Technology Vol. 97, 1780-1787 (2006). http://dx.doi.org/10.1016/j.biortech.2005.09.009

B. Preetha and T. Viruthagiri, “Batch and continuous biosorption of chromium (VI) by Rhizopus arrhizus,” Separation Purification Technology 57, 126-133 (2007). http://dx.doi.org/10.1016/j.seppur.2007.03.015

J. Srivastava, H. Chandra, K. Tripathi, R. Naraian and R.K. Sahu, “Removal of chromium (VI) through biosorption by the Pseudomonas sp. isolated from tannery effluent” Journal of Basic Microbiology 48, 135-139 (2008). http://dx.doi.org/10.1002/jobm.200700291

A.Y. Dursun, G. Uslu, Y. Cuci and Z. Aksu, “Bioaccumulation of copper (II), lead (II) and chromium (VI) by growing Aspergillus niger” Process Biochemistry 38 (12), 1647-1651 (2003). http://dx.doi.org/10.1016/S0032-9592(02)00075-4

G. Resmi, S.G. Thampi and S. Chandrakaran, “Brevundimonas vesicularis: A Novel Biosorbent for Removal of Lead from Wastewater” International Journal of Environmental Research 4 (2), 281-288 (2010).

R. Faryel, A. Sultan, F. Tahir, S. Ahmed and A. Hameed, “Biosorption of lead by indigenous fungal strains” Pakistan Journal of Botany 39(2), 615-622 (2007).

W. Jianlong, Z. Xinmin, D. Decaib and Z. Dingc, “Bioadsorption of lead (II) from aqueous solution by fungal biomass of Aspergillus niger” Journal of Biotechnology 87(3), 273-277 (2001). http://dx.doi.org/10.1016/S0168-1656(00)00379-5

P. Baldrian, J. Gabriel and F. Nerud, “Effect of cadmium on the ligninolytic activity of Stereum hirsutum and Phanerochaete chrysosporium” Folia Microbiologica, 41, 363–367 (1996). http://dx.doi.org/10.1007/BF02814716

A. Hassen, N. Saidi, M. Cherif and A. Boudabous, “Effects of heavy metals on Pseudomonas aeruginosa and Bacillus thurengiensis” Bioresource Technology 65, 73-82 (1998). http://dx.doi.org/10.1016/S0960-8524(98)00011-X

E. Parameswari, A. Lakshmanan and T. Thilagavathi, “Chromate Resistance and Reduction by Bacterial Isolates” Australian Journal of Basic and Applied Sciences 3 (2), 1363-1368 (2009).

M. Vasanthy, “An investigation on removal of chromium (VI) using bacterial strains” Asian Journal of Microbiology, Biotechnology and Environmental Sciences 6 (4), 583586 (2004). [43] A. Zahoor and A. Rehman, “Isolation of Cr (VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater” Journal of Environmental Sciences 21 (6) 814–820 (2009). http://dx.doi.org/10.1016/S1001-0742(08)62346-3

M. Nourbakhsh, Y. Sag, D. Ozer, Z. Aksu, T. Kutsal and A. Caglar, “A comparative study of various biosorbents for removal of chromium(VI) ions from industrial waste waters’ Process Biochemistry 29, 1-5 (1994). http://dx.doi.org/10.1016/0032-9592(94)80052-9

U. Yestis, G. Ozcengiz, F.B. Dilek, N. Ergen, A. Erbay and A. Dolek, “Heavy metal biosorption by white-rot fungi,” Water Science and Technology, 38(4-5), 323-330 (1998). http://dx.doi.org/10.1016/S0273-1223(98)00515-0

H. Niu, X. S. Xu, and J.H. Wang, “Removal of lead from aqueous solutions by Penicillin biomass,” Biotechnology and Bioengineering, 42, 785-787 (1993). http://dx.doi.org/10.1002/bit.260420615

R. Say, A. Denizli and A. M. Yakup, “Biosorption of cadmium (II), lead (II) and copper (II) with the filamentous fungus Phanerochaete chrysosporium,” Bioresource Technology 76, 67-70 (2001). http://dx.doi.org/10.1016/S0960-8524(00)00071-7

T. Akar and S. Tunali, “Biosorption performance of Botrytis cinerea fungal by-products for removal of Cd (II) and Cu (II) ions from aqueous solutions,’ Minerals Engineering 18, 1099-1109 (2005). http://dx.doi.org/10.1016/j.mineng.2005.03.002