Receptor Binding Domain (RBD) Structural Susceptibility in the SARS-CoV-2 Virus Spike Protein Exposed to a Pulsed Electric Field

  • D. Osorio-González Molecular Biophysics Laboratory of the Faculty of Sciences, Autonomous University of the State of Mexico, Mexico; Center for Research, Technology Transfer and Business Innovations. CITTIES. México https://orcid.org/0000-0003-1636-6317
  • V. J. Muñiz-Orozco Molecular Biophysics Laboratory of the Faculty of Sciences, Autonomous University of the State of Mexico, Mexico https://orcid.org/0000-0003-3136-650X
  • C. P. González Molecular Biophysics Laboratory of the Faculty of Sciences, Autonomous University of the State of Mexico, Mexico https://orcid.org/0000-0002-0479-1861
  • M. Fuentes-Acosta Molecular Biophysics Laboratory of the Faculty of Sciences, Autonomous University of the State of Mexico, Mexico; Center for Research, Technology Transfer and Business Innovations. CITTIES, México https://orcid.org/0000-0002-6468-7978
  • J. Mulia-Rodríguez Molecular Biophysics Laboratory of the Faculty of Sciences, Autonomous University of the State of Mexico, Mexico https://orcid.org/0000-0002-5521-7421
  • L. A. Mandujano-Rosas Molecular Biophysics Modeling and Prototyping Laboratory, Mexiquense University, S. C., State of Mexico, Mexico; Center for Research, Technology Transfer and Business Innovations. CITTIES, México https://orcid.org/0000-0001-9396-7402
Keywords: SARS-CoV-2, COVID-19, Receptor-Binding Domain (RBD)

Abstract

SARS-CoV-2 is responsible for causing the Coronavirus disease 2019 (COVID-19) pandemic, which has so far infected more than thirty million people and caused almost a million deaths. For this reason, it has been a priority to stop the transmission of the outbreak through preventive measures, such as surface disinfection, and to establish bases for the design of an effective disinfection technique without chemical components. In this study, we performed in silico analysis to identify the conformational alterations of the SARS-CoV-2 Spike Receptor Binding Domain (RBD) caused by the effect of a pulsed electric field at two different intensities. We found that both stimuli, especially the one with the highest angular frequency and amplitude, modified the electrical charge distribution in the RBD surface and the number of hydrogen bonds. Moreover, the secondary structure was significantly affected, with a decrease of the structured regions, particularly the regions with residues involved in recognizing and interacting with the receptor ACE2. Since many regions suffered conformational changes, we calculated RMSF and ΔRMSF to identify the regions and residues with larger fluctuations and higher flexibility. We found that regions conformed by 353-372, 453-464, and 470-490 amino acid residues fluctuate the most, where the first is considered a therapeutic target, and the last has already
been characterized for its flexibility. Our results indicate that a pulsed electric field can cause loss of stability in the Spike-RBD, and we were able to identify the vulnerable sites to be used as a starting point for the development of viral inhibition or inactivation mechanisms.

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Published
2021-02-10
How to Cite
(1)
Osorio-González, D.; Muñiz-Orozco, V. J.; González, C. P.; Fuentes-Acosta, M.; Mulia-Rodríguez, J.; Mandujano-Rosas, L. A. Receptor Binding Domain (RBD) Structural Susceptibility in the SARS-CoV-2 Virus Spike Protein Exposed to a Pulsed Electric Field. J. Nucl. Phy. Mat. Sci. Rad. A. 2021, 8, 177-182.
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